Simultaneous quantification of THC-COOH, OH-THC and further cannabinoids in human hair by GC-MS/MS with electron ionisation applying automated sample preparation.
Drug Test Anal. 2018 Aug 24;:
Authors: Kieliba T, Lerch O, Andresen-Streichert H, Rothschild MA, Beike J
The detection of Δ9 -tetrahydrocannabinol (THC), cannabidiol (CBD) and cannabinol (CBN) in hair, for the purpose of identifying cannabis consumption, is conducted in many forensic laboratories. Since external contamination of hair with these cannabis components cannot be excluded, even after hair decontamination, only the detection of THC metabolites such as 11-nor-9-carboxy-Δ9 -tetrahydrocannabinol (THC-COOH) or 11-hydroxy-Δ9 -tetrahydrocannabinol (OH-THC), is considered to prove cannabis consumption. At present, testing for THC metabolites is not standard practice due to its analytical complexity. For these reasons, we developed a novel method for the detection of THC-COOH and OH-THC as well as THC, CBD and CBN in one single analytical run using gas chromatography-tandem mass spectrometry (GC-MS/MS) with electron ionisation. After manual hair washing and grinding, sample preparation was fully automated, by means of a robotic autosampler. The hair extraction took place by digestion with sodium hydroxide. A solid phase extraction (SPE) was chosen for sample clean-up, using a mixed-mode anion exchange sorbent. Derivatisation of all analytes was by silylation. The method has been fully validated according to guidelines of the Society of Toxicological and Forensic Chemistry (GTFCh), with a limit of detection (LOD) of 0.2 pg/mg for THC-COOH and OH-THC and 2 pg/mg for THC, CBD and CBN, respectively, thus fulfilling the Society of Hair Testing (SoHT) recommendations. The validated method has been successfully applied to our routine forensic case work and a summary of data from authentic hair samples is given, as well as data from proficiency tests.
PMID: 30144294 [PubMed – as supplied by publisher]
Source: Estudios sobre Cannabidiol (CBD)